Quantitative Tests For Aminoacids And Proteins Biology Essay

Quantitative Tests For Aminoacids And Proteins Biology Essay The purpose of this experiment is to obtain information about structural of a compound with some tests these are biuret ,ninhydrine ,xanthoprotic and heavy metal group by observing colour changes or not. Casein is a protein that is found in milk and used as a binding agent. Technically, phosphoproteins, collections of proteins bound to something containing phosphoric acid.[1] Albumin is an umbrella term for a type of protein which is water soluble. Albumin can be found two of the most familiar examples that are in egg whites and in human blood. Albumins are an important class of protein, and they are vitally important to health and well being for many organisms. Many plants and animals contain or secrete albumin. [2] Tyrosine is a nonessential amino acid the body makes from another amino acid called phenylalanine. It is a building block for several important neurotransmitters, serotonin, dopamine etc. Neurotransmitters help nerve cells communicate and influence mood. Tyrosine also helps produce melanin (the pigment responsible for hair and skin color) and helps in the function of organs responsible for making and regulating hormones. It is involved in the structure of almost every protein in the body.[3] Protein Testing: Biuret Test: The Biuret Test is a general test to detect peptide bond or not. When protein that includes peptide bond reacts with copper(II) sulfate (blue), the positive test is the formation of a violet colored complex. The Biuret Test works for any compound containing two or more of the following groups. Ninhydrin Test: The Ninhydrin Test is a test for amino acids and proteins to detect free -NH2 group. When such an -NH2 group reacts with ninhydrin, a purple-blue complex is formed. Xanthproteic Test: Phenyl group containing an activating group can be nitrated it produces as a yellow product. The production of a yellow colored product provide by the addition of nitric acid and this test works for the presence of tyrosine or tryptophan in a protein.The addition of strong base will deepen the color to orange. The yellow stains on the skin caused by nitric acid are the result of the xanthoproteic reaction. Heavy Metal Ions Test: Heavy metal ions precipitate proteins by cross-linking free amino groups and carboxylate groups. Ions commonly used for testing for the presence of proteins include Zn2+, Fe3+, Cu2+, Sb3+, Ag1+, Cd2+, and Pb2+. Among the metal ions, Hg2+, Cd2+, and Pb2+ have very high toxicity. They cause serious damage to proteins (especially enzymes) by denaturing them. [4] APPARATUS Dropper Test tubes Heater %2Casein %2Albumin %2Gelatin %2Glycine %2Trypthophan NaOH Copper sulfate Ninhydrine reagent Nitric acid Milk Silver nitrate Iron nitrate Sodium nitrate Water Bath PROCEDURE Biuret Test Firstly,%2 solutions ;glycine,gelatin,albumin,thyrosine and casein were prepared. These tubes were labelled as 1,2,3,4 and 5 1ml of each solution except casein and put caserin with 1 ml water. 7-10 drops NaOH and 2 drops copper sulfate solution on each of the samples to be tested. Observations were recorded. Ninhydrine Test New test tubes were labelled again. 7-10 drops of ninhydrin reagent were added to each test tube and heated in water bath approximately 5 min. Observations were recorded. Xanthoproteic Test New test tubes were labelled 10-15 drops of nitric acid were added to each test tube and heated in water bath approximately 10min. Observations were recorded. Heavy Metal Precipitation 1.2ml milk were put into glycine ,gelatine and albumin labelled as 1,2,3 2.10 drops of %5silver nitrate were added to glycine ,10 drops of iron(III) nitrate to gelatine and 10 drops of sodium nitrate to test tube albumin. 3.These steps were repeated for %2 albumin. 4.Finally,observations were recorded. OBSERVATIONS In this experiment,we placed new test tubes into rack by labelled each with number.The reason of that is to apply some tests to each one.These tests are biuret ,ninhydrine ,xanthoproteic and heavy metal tests.Casein,albumin ,gelatin ,glycine and tyrosine were tested with biuret test.Firstly inside blue layer with casein and then light purple at top of tube and blue layer.In albumin,the purple colour diffused in test tube ,and then purple at top and purple layer at bottom. With gelatin ,at top is purple as a first and then purple particles were also observed.For glycine ,blue appeared as homogeneous like dissolved.And then at top is blue ,at bottom like green.Final result for biuret test is for tyrosine.At the beginning, blue layer was remained in suspense and then green ,brown particle was observed at bottom. Secondly,ninhydrine test results were recorded for each one.With casein,we observed light purple like grey.With gelatin ,glycine and also tyrosine dark purple.With albumin,the color appeared like dark purple due to heater. Thirdly,the other test is xanthoprotic test to detect easily phenyl group.With casein ,opaque turned into yellow particles.With gelatin and glycine ,the colour is so light yellow like colourless.With tyrosine ,yellow color turned into brown in time.Finally,albumin appeared as cloudy white and then turned into yellow particles. Final test is heavy metal test that gives information about structure.Milk with silver nitrate turned into pink due to density.With iron and sodium nitrate ,there are no changes. RESULTS In this experiment, we learned some test to obtain information that we searched.For example,Biuret test is help to give information about peptide bond or not.If there is a peptide bond ,the color is observed as purple.If the colour is blue,it means no rxn there.Casein ,albumin ,gelatin,glycine and tyrosine were tested.Casein,albumin and gelatin gave purple color and these ones have peptide bond.Because these are proteins that have peptide bonds.However, glycine and tyrosine are not proteins one of them is aminoacid the other is enzyme.Therefore they did not give rxn not observed purple color as we expected. With ninhydrine test,except casein,the others gave pozitive rxn appeared as purple color.This test is to help information about free amino group primary or secondary.The other test is xantroprotic test.It gives information about phenyl group.Aromatic group one is yellow.Nitric acid is used to test it.This reaction is also endothermic rxn.Casein and tyrosine gave reaction and observed color changes.The final test is heavy metal test.It helps to give information about structure of a compound.If protein is tested with heavy metal,colour changes are observed also.It is tested with silver ,iron and sodium nitrate also.Silver helps to distrupt the structure that means colour change.With silver,the color turns into pink. DISCUSSION The purpose of this experiment is to learn which test why is used.Biuret test is to detect there are peptide bond or not.Normally,biuret test can give pozitive result that means purple color with proteins.In our experiment,casein albumin and gelatin give (+) result however glycine and tyrosine not due to aminoacid or enzyme.Our results are also related to as we expected.Ninhydrine test was applied to same prepared samples.The aim of this test is to detect free aminogroup (-NH2) and to decide primary or secondary group according to the colour.We used heater in this part.The reason of that reaction become a reality due to endothermic.At this part,except casein the others could give (+)result.Glycine that we learned previous experiment can have free aminogroup (primary).With casein,the others colour turned into dark purple. The other test is xandroprotic test that is used to detect phenly group.It helps to discriminate aromatic structure from others.For casein ,the colour turned into yellow,for tyrosine ,the colour turned yellow to orange like brown.Therefore ,two of them could give pozitive result.As a final test ,heavy metal test was applied to prepared samples.This test shows which sample can distrupt with which one.Silver ,iron and sodium nitrate were used to detect it.Silver can distrupt protein structure.We understood that colour changes.The color turns into pink and the others did not change for milk (nesquick).

Methods of Collecting Data in HRM

The process of personnel selection includes examination of personal and professional qualities of a prospective employee in order to determine his/her abilities to correspond to the requirements of one or another position. As a rule, personnel selection is a long and costly procedure, that’s why in many organizations it is performed in several stages. The first stage usually involves collecting different data about the candidates for the opening. There are some popular methods, which help to gather such information for further analysis. The first group of the methods includes collecting information about candidate’s personal qualities and experience. Overwhelming majority of organizations asks the candidates to submit their CVs, to fill special forms or application blanks, which can provide the employers with all necessary biographical information about potential employees. Besides, one of the most effective and popular methods to examine personal qualities, appearance and responses of a candidate on different situations, is interview. This method is very flexible and informative. But, at the same time, it is usually hard to avoid subjectivity when using this technique. The second group of these methods includes different tests, which are mostly directed on examination of professional qualities of candidates. Personality test is a method, which allows to measure personal qualities of a candidate in relation to the requirements of the workplace.   A number of aptitude and cognitive abilities tests give opportunity to measure mental abilities and intellectual level of the applicants. Such tests as physical abilities test and work sample tests help to discover physical readiness of a candidate to perform one or another job. Effectiveness of the above mentioned methods depends, first of all, on the specificity of the job, as well as on mission of the organization. If the job is connected with physical activity, like a worker or a loader, then physical ability or work sample tests can be effective. If an employer is primarily interested in personal qualities of a candidate, such methods as interview or personality test can give the best results. Besides, it is always necessary to keep in mind that the information collected with the help of the tests or interviews can be subjective to some extent, because it is hard to avoid subjectivity in any evaluation, made by a human being. Bibliography: Personnel Selection: Overview. (2001). HR-Guide. Human Resourse Guide on Internet. Retrieved November 12, 2006, from the World Wide Web: .   

Adaptation Mcdonalds

McDonald's Going International International Marketing Mag. K. Roth Group 2 Florian Antos Vladimir Slon Christoph Gluszko 1 Christian Schromm ALL YOU WANTED TO KNOW ABOUT McDonald's 2 History †¢ McDonald's Corporation – is the world's largest chain of fastfood restaurants, primarily selling hamburgers, chicken, French fries, milkshakes and soft drinks. More recently, it also offers salads, fruit and carrot sticks. The business began in 1940, with a restaurant opened by Dick and Mac McDonald in San Bernardino, California.Their introduction of the â€Å"Speedee Service System† in 1948 established the principles of the modern fast-food restaurant. The present corporation dates its founding to the opening of a franchised restaurant by Ray Kroc, in Illinois on April 15, 1955, the ninth McDonald's restaurant overall. Kroc later purchased the McDonald brothers' equity in the company and led its worldwide expansion. 3 †¢ †¢ †¢ †¢ What are the advantages and disadvantages of adaptation strategy used by McDonald’s? 4 1.Advantages and Disadvantages of adaptation strategy Advantages: †¢ Different cultures have different consumer needs and tastes †¢ Political and legal environment of the international market that they plan to enter †¢ Differences among nations in terms of cultures, religious believes, political and legal systems, customer values and lifestyles, and stages of economic and market development †¢ Differences in the advancement of technology †¢ Competition plays a major role in determining if a company will adopt a product adaptation scheme 1. Advantages and Disadvantages of adaptation strategy Disadvantages: †¢ The costs associated with adaptation can also play a major role in determining whether or not a firm chooses to standardize their product 6 1. Advantages and Disadvantages of adaptation strategy †¢ †¢ †¢ Cross any boarder and you will find multiple variations from price to product, starting with the presentation. Misconception that McDonalds is the same everywhere.Country teams have considerable autonomy to develop and market new product lines. Menu differences: Netherlands and Austria– Beer Germany – Chinese Week Canada – Mc Pizza India – Goat and lamb burgers half of menu vegetarian Japan – ingredients such as cabbage and Teriyaki USA – Barbecue bacon Burgers, sausage breakfast burritos 7 Are there examples of truly global brands that never customized? 8 Are there examples of truly global brands that never customized?Truly global Brands : †¢ In general we can say that straight extension (=standardization) has been implemented and already been successful with cameras, consumer electronics, and many machine tools. Stihl ( machinery and chainsaw producer) Nikon Olympus, Canon ( camera producers) Kitchen Aid (consumer electronics ) Caterpillar (trucks, bulldozers) †¢ †¢ †¢ †¢ 9 What have been the key factors that have led to McDonald’s global success? 10 2. What have been the key factors that have led McDonalds to global success? †¢ †¢ †¢ Fast service enabled by a limited menu, focus on cleanliness, family friendly facilities, good value for money Emergence of cash-rich, time-poor lifestyle Societal acceptance of a more casual approach to eating on the run underscored the emergence of McDonalds Innovations in food preparation technology and service delivery providing a fast- service format that customers appreciate as well as a cost base that competitors found hard to match Waiter labor cost savings passed on directly to the consumers through lower prices McDonalds was the leader to introduce franchising in the global marketplace, augmenting the organic restaurant growth by harnessing the management, cultural and entrepreneurial capabilities and capital of local businesspeople around the world.Expanding across the globe Increasing res taurant penetration in already existing markets †¢ †¢ †¢ 11 2. What have been the key factors that have led McDonalds to global success? Key Faktors: †¢ †¢ †¢ †¢ †¢ †¢ Economies of scale in production and distribution Power and scope Brand image was country suited Ability to leverage ideas quickly and efficiently Uniformity of marketing practices Pervasiveness of McDonalds brand 12 2. What have been the key factors that have led McDonalds to global success? †¢ †¢ †¢ †¢ †¢ McDonald's is seen as the number one representative of the all American lifestyle, which at the time when the expansion began was very popular. In some countries it is still a status symbol to go and have a meal at McDonald's.Generally the cooperation is at pains to offer a selection of standardized core products ( Big Mac, Soft drinks, French Fries) in addition to locally accepted alternatives. Giving the multinational cooperation a local Face ( thinking of Salzburg's restaurant) In order to use synergies on the one hand and reduce national competences on the other a supranational administrative department had been set up in Europe. McDonald's. has always been supported by US foreign policy Sources : http://de. wikipedia. org/wiki/McDonald’s; Kotler/Keller Marketing Management 13 What are the risks and benefits of choosing the franchising system for franchisor? 14 3. Risks and Benefits of franchise system Benefits for franchisor: The franchisor profits from the strongly motivated franchisee as a self-dependent entrepreneur †¢ The franchising as a way to overcome the resource scarcity through the direct market access †¢ The ability to overcome the high costs of arranging the new distribution network through using the well tailored network of franchisor †¢ Low capital investments for the product placement and region marketing through the franchisee †¢ The benefits from using a recognized brand name and trade marks through the reputation of the franchisor †¢ Extended expansion possibility through franchising 15 3. Risks and Benefits of franchise system Risks for franchisor: †¢ The existence of multiple, geographically dispersed owner-managers makes the control difficult †¢ Possible high costs of training development, promotional and support activities of franchisee †¢ Service delivery may be inconsistent because of geographically dispersed franchisees 16 What are the key issues by setting up a McDonald’s Franchisee? 17 4. Risks and Benefits of franchise system Benefits for the franchisee: †¢ Your business is based on a proven idea.You can check how successful other franchises are before committing yourself. †¢ You can use a recognized brand name and trade marks. You benefit from any advertising or promotion by the owner of the franchise – the â€Å"franchisor†. †¢ The franchisor gives you support – usually including training, help setting up the business, a manual telling you how to run the business and ongoing advice. †¢ Financing the business may be easier. Banks are sometimes more likely to lend money to buy a franchise with a good reputation. †¢ Risk is reduced and is shared by the franchisor. †¢ If you have an existing customer base you will not have to invest time looking to set one up. Relationships with suppliers have already been established. †¢ The ability to overcome the resource constrains. 18 4. Risks and Benefits of franchise system Risks for franchisee: †¢ Costs may be higher than you expect. As well as the initial costs of buying the franchise, you pay continuing royalties and you may have to agree to buy products from the franchisor. †¢ The franchise agreement usually includes restrictions on how you run the business. You might not be able to make changes to suit your local market. †¢ The franchisor might go out of business, or change the way they do things. †¢ Other franchisees could give the brand a bad reputation. You may find it difficult to sell your franchise – you can only sell it to someone approved by the franchisor. 19 4. Costs and Restrictions Costs: Financial Requirements/Down Payment : Initial down payment for opening (40% of the total cost) or an existing restaurant (25% of the total cost) about 200. 000 $ nonborrowed resources Financing: McDonald’s does not offer financing; McDonald’s Owner/Operators enjoy the benefits of our established relationships with many national lending institutions 20 4. Costs and Restrictions Restrictions: During the term of the franchise, you pay McDonald’s the following fees: †¢ Service fee: a monthly fee based upon the restaurant’s sales performance (currently a service fee of 4. 0% of monthly sales). Rent: a monthly based rent or percentage rent that is a percentage of monthly sales. †¢ Application: Personal, non-borrowed res ources to be invested in a McDonald's restaurant business. †¢ Business experience in the market where they are seeking a franchise 21 What strategy would you adopt to ensure a great exposure for McDonald’s to a growing trend towards healthier eating? 22 5. Healthier eating at McDonald’s Currently: †¢ †¢ Low fat products in UK outlet Canadian light choices of Menu with a Soya bean McVeggie Burger, salads with a fat free dressing and a granola topped fruit yoghurt On packaging calorie and fat content information for the first time as it tries to counter the obesity lobby †¢ 23 5.Healthier eating at McDonald’s Future perspectives: †¢ †¢ †¢ †¢ †¢ †¢ Using biologically grown resources Fat reduced meals Healthier ways of processing the food (eg. : don’t fry chips but bake them, less white bread burgers, regular vegetarian dishes,) Healthy, freshly squeezed Mc Shakes etc. Healthier Drinks not only Sodas To ensure high-quality, fresh ingredients, farms are set up in order to supply restaurants 24 Sources: †¢ †¢ †¢ †¢ †¢ www. McDonalds. com www. freeforessays. com/show_essay/55461. html D. Jobber â€Å"Principles and Practice of Marketing† Sources : http://de. wikipedia. org/wiki/McDonald’s Kotler/Keller Marketing Management 25 THANK YOU FOR YOUR ATTENTION 26

Biography of Thomas Cole, American Landscape Painter

Thomas Cole was the British-born artist who became known for his paintings of American landscapes. He is considered the founder of the Hudson River School of painting, and his influence on other 19th century American painters was profound. Cole’s paintings, and paintings by those he taught, are known to have influenced attitudes toward American expansionism during the 19th century. The glorification of the land and the panoramic views encouraged optimism toward settling the vast lands of the West. Cole, however, had a pessimistic streak which is sometimes indicated in his paintings. Fast Facts: Thomas Cole Known For: Founder of Hudson River School of painters, admired for his majestic landscapes of distinctly American sceneryMovement: Hudson River School (American romantic landscape painting)Born: Bolton-le-Moors, Lancaster, England, 1801Died: February 11, 1848 in Catskill, New YorkParents: Mary and James ColeSpouse: Maria Bartow Early Life and Career Thomas Cole was born in Bolton-le-Moors, Lancaster, England, in 1801. He studied engraving briefly in England before emigrating to America with his family in 1818. The family arrived in Philadelphia and resettled in Steubenville, Ohio, where Coles father established a wallpaper engraving business. After becoming frustrated working in the family business, Cole taught art in a school for a brief period. He also received some painting instruction from a traveling artist, and tried striking out on his own as an itinerant portrait painter. Portrait of Thomas Cole, American painter. Smithsonian Institution / Wikimedia Commons / Public Domain Cole realized he needed to be in a city with many potential patrons, and returned to Philadelphia, where he painted portraits and also found work decorating ceramics. He took classes at the Philadelphia Academy and, in 1824, had his first exhibition, which was held at the school. In 1825 Cole moved to New York City, where he began to focus on romantic landscapes, the beautifully lit panoramas that would become his enduring style. After taking a trip up the Hudson River, he painted three landscapes, which were displayed in the window of a Manhattan art store. One of the paintings was purchased by artist John Trumbull, who was widely known for his paintings of the American Revolution. Trumbull recommended that two of his artist friends, William Dunlap and Asher B. Durand, purchase the other two. Trumbull appreciated that Cole had been inspired by the wildness of American scenery, which other artists had seemed to ignore. On Trumbulls recommendation, Cole was welcomed into New York Citys cultural world, where he became acquainted with luminaries such as poet and editor William Cullen Bryant and author James Fenimore Cooper. Travels and Inspiration The success of Cole’s early landscapes established him so he could devote himself to painting full-time. He began to travel in the mountains of New York State and New England after purchasing a house in Catskill, New York. Photograph of Catskill Mountain House, a painting by American landscape artist Thomas Cole. Francis G. Mayer  / Getty Images In 1829 Cole sailed to England on a trip financed by a wealthy patron. He made what was known as the Grand Tour, visiting Paris, and then Italy. He stayed for weeks in Florence before going on to Rome, hiking much of the way. He eventually returned to New York City in 1832, having seen major works of art in Europe and having sketched scenery that would be used as material for landscapes. In 1836 Cole married Maria Barton, whose family lived in Catskill. He settled into a fairly comfortable life as a successful artist. The self-made gentry of the region admired his work and purchased his paintings. Major Works A patron commissioned Cole to paint five panels which would be known as The Course of Empires. The series of canvases essentially predicted what would become known as Manifest Destiny. The images depict an allegorical empire, and proceed from Savage State to Arcadian or Pastoral State. The empire reaches its zenith with the third painting, The Consummation of Empire, and then descends to the fourth painting, Destruction. The series ends with the fifth painting, titled Desolation. Thomas Coles The Course of Empire - Consummation, 1836, oil on canvas, 51 Ãâ€" 76 in, New York Historical Society.   Fine Art / Getty Images During the 1830s, as Cole was painting his Course of Empires series, he was harboring gravely pessimistic thoughts about America, lamenting in his journal that he feared the end of democracy. One of his major paintings, dating from 1836, is titled View from Mount Holyoke, Northampton, Massachusetts, after a Thunderstorm — The Oxbow. In the painting, a pastoral area is shown along with a portion of untamed wilderness. On close examination, the artist himself can be found in the middle foreground, on a promontory, painting the Oxbow, a bend in the river. In his own painting, Cole looks out over the tamed and orderly land, yet he is located in the wild land which is still darkened from the passing storm. He shows himself in communion with untamed American land, perhaps purposely keeping a distance from the land which has been transformed by human society. View from Mount Holyoke, Northampton, Massachusetts, After a Thunderstorm -- The Oxbow.   Getty Images Legacy Interpretations of Coles work have varied over time. On the surface, his works are generally appreciated for their majestic scenes and striking use of light. Yet there are often darker elements present, and many paintings have dark areas which seem to raise questions about the artists intent. Coles paintings show a profound reverence for nature, which can appear idyllic or wild and violent within the boundaries of the same canvas. While still a very active artist, Cole became ill with pleurisy. He died on February 11, 1848. His influence on other American painters was profound. Sources Thomas Cole. Encyclopedia of World Biography, 2nd ed., vol. 4, Gale, 2004, pp. 151-152. Gale Virtual Reference Library.Hudson River School of Painting. American Eras, vol. 5: The Reform Era and Eastern U. S. Development, 1815-1850, Gale, 1997, pp. 38-40. Gale Virtual Reference Library.The Hudson River School and Western Expansion. American Eras, vol. 6: Westward Expansion, 1800-1860, Gale, 1997, pp. 53-54. Gale Virtual Reference Library.